The task of the Bioinformatics Research Group of Szentágothai Research Centre of the University of Pécs is to perform the new generation sequencing processes in the National Laboratory of Human Reproduction. The team performs nucleic acid isolation from biological samples (e.g., nutrient solutions, breast milk, saliva, blood, follicular fluid), creation of new generation sequencing libraries, sequencing, microarray-based studies, and bioinformatics analysis of the resulting data, and in addition, participates in the statistical evaluation and interpretation of the results. The PCR laboratory of the research group related to clinical molecular laboratory diagnostics performs molecular analysis of various human samples (blood, nutrient solutions, saliva, follicular fluid) using real-time quantitative PCR and droplet digital PCR methods. Some of the samples are fetal culture fluids left over from artificial insemination procedures (IVF, ICSI) from the Department of Obstetrics and Gynecology of the University of Pécs and the IVF
Center of the National Laboratory for Human Reproduction, from which nucleic acid determination is performed; nucleic acid extraction, amplification, determination of microRNAs present in the sample by droplet digital PCR method. The aim of the research group is to identify the molecular differences that affect fertility and to predict the viability and implantability of embryos based on molecular genetic markers obtained from embryonic media. Following non-invasive molecular risk assessment strategies, we would like to increase the effectiveness of assisted reproduction techniques using our methods.